Ixmyelocel-T co-culture results in increased endothelial nitric oxide synthase and nitric oxide production in endothelial cells. (A) Immunofluorescence of endothelial nitric oxide synthase (eNOS) in co-cultured human umbilical vein endothelial cells (HUVECs) (n ≥3). (B) eNOS protein (n ≥11). (C) Nitric oxide (NO) generation (n ≥9). (D) Nitrates measured in co-culture supernatants (n ≥8). Values presented as mean ± standard error of the mean relative to control. *P <0.05, **P <0.01, ***P <0.001 versus HUVECs. Magnification: 20×. IXT, ixmyelocel-T.