Figure 4

Co-culture with ixmyelocel-T decreases apoptosis, oxidative stress, and inflammation in TNFα pretreated endothelial cells. (A) Apoptosis (n = 6). (B) Viability was decreased with ixmyelocel-T co-culture (n = 6). (C) Ixmyelocel-T co-culture decreased reactive oxygen species (ROS) generation (n ≥6). (D) Ixmyelocel-T co-culture increased antioxidant superoxide dismutase (SOD) in human umbilical vein endothelial cells (HUVECs) after tumor necrosis factor alpha (TNFα) stimulation (n ≥4). Protein expression of (E) pNFκB, (F) pIKBa, and (G) pIKKa were all significantly decreased in HUVECs co-cultured with ixmyelocel-T after TNFα stimulation (n = 3). Values presented as mean ± standard error of the mean relative to control. *P <0.05, **P <0.01, ***P <0.001 versus HUVECs + TNFα. IXT, ixmyelocel-T.