Analysis of PKH26-labeled ixmyelocel-T in the hind-limb ischemia model. (A) High magnification of labeled ixmyelocel-T (green) throughout the ischemic limb 8 days after initiation of hind-limb ischemia. Sections were immunostained for type I collagen (purple) and counterstained with 4′-6-diamidino-2-phenylindole (Blue). (B) Analysis of ixmyelocel-T (red) co-localization with human nuclear antigen (green) in the ischemic rodent limb. Magnification: 400×. HNA, human nuclear antigen; IXT, ixmyelocel-T.