Figure 4

Keratinocyte-conditioned medium induced the expression of the keratinocyte-specific markers cytokeratin-14 and cytokeratin-5. (A) Bone marrow mesenchymal stem cells (BMSCs) from different induction media and time points (days 0, 7, 14, and 21) were co-labelled with cytokeratin-14 and cytokeratin-5. DAPI (4,6-diamidino-2-phenylindole) was used as a counterstain. Keratinocytes at passages 2 to 5 and BMSCs at passages 4 to 7 were used as positive and negative controls, respectively. Scale bar: 50 μm. (B) The average number of positively stained cells divided by the average total number of cells (nuclei counts) and multiplied by 100 to obtain the percentage. Data are presented as the mean ± standard error of the mean. n = 3 slides per time point. **P <0.01 compared with the relative value at day 0, ^# P <0.05, ^## P <0.01 KCM1 versus KCM2. KCM1, keratinocyte-conditioned medium collected from keratinocytes with no Y-27632 treatment; KCM2, keratinocyte-conditioned medium collected from keratinocytes treated with Y-27632.