Immunofluorescent analysis of stratifin and involucrin 21 days after the application of different keratinocyte-conditioned media. (A) Bone marrow mesenchymal stem cells (BMSCs) were co-immunostained with stratifin and involucrin in two conditions. DAPI (4,6-diamidino-2-phenylindole) was used as a counterstain. Keratinocytes at passages 2 to 5 and BMSCs at passages 4 to 7 were used as positive and negative controls, respectively. Scale bar: 50 μm. (B) The average number of positively stained cells divided by the average total number of cells (nuclei counts) and multiplied by 100 to obtain the percentage. Data are presented as the mean ± standard error of the mean. n = 3 slides per group. **P <0.01 BMSCs versus KCM1/KCM2, #
P <0.05 KCM1 versus KCM2. KCM1, keratinocyte-conditioned medium collected from keratinocytes with no Y-27632 treatment; KCM2, keratinocyte-conditioned medium collected from keratinocytes treated with Y-27632.