MSC differentiation was maintained in the presence of pullulan. Osteogenesis, demonstrated by alizarin red stain showed increased mineral deposition in 2% and 5% pullulan-treated groups as compared to the 0% control. Oil Red O staining of lipid droplets for adipogenesis showed differentiated MSCs in all pullulan-treated groups. Histological assessment by Safranin-O of MSCs in pellet chondrogenic culture showed GAG deposition in all pullulan-treated groups. GAG, glycosaminoglycans; MSCs, mesenchymal stem cells.