Figure 6
Ex vivo expansion of CD34 + CD133+ cells on MenSCs-feeder shows high potential of differentiation toward hematopoietic cells. In order to evaluate the progenitors of the hematopoietic lineage, CFU assay was performed by plating a single cell suspension in a semi-solid MethoCult™ media. At 14 days post-culture, colony types derived from the initial equivalent of 50 cord blood HSC was classified and counted according to morphological criteria. (A) Total number of CFU-GEMM, CFU-GM, and BFU-E. Expansion of HSCs on the MenSCs-feeder have a higher capacity to generate CFU-GEMM compared to the other culture conditions. Values are expressed as mean ± SE. (**P ≤0.01). (B) Phase-contrast microscopy of representative CFU-GEMM, CFU-GM and BFU-E colonies. Images are representative of each condition. Original magnification x10. Data shown are representative of multiple replicates. BFU-E, burst-forming unit-erythroid; CFU-GEMM, colony-forming unit-granulocyte, erythroid, macrophage and megakaryocyte; CFU-GM, colony-forming unit-granulocyte and macrophage; HSCs, hematopoietic stem cells; SE, standard error.