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Figure 5 | Stem Cell Research & Therapy

Figure 5

From: Culture of human mesenchymal stem cells using a candidate pharmaceutical grade xeno-free cell culture supplement derived from industrial human plasma pools

Figure 5

Osteogenic differentiation. Illustrative micrographs of human mesenchymal stem cells (hMSCs) cultured with commercial medium and with Basal Medium 2 with 15% supplement for cell culture (SCC), showing osteogenic differentiation. Alizarin Red S staining revealed extracellular calcium deposits. Alkaline phosphatase activity was present in the differentiated cells, which was shown by 5-bromo-4-chloro-3'-indolyl phosphate p-toluidine salt and nitro-blue tetrazolium chloride (BCIP/NBT tablet, Ref. B5655-5TAB; Sigma-Aldrich, St. Lous, MO, USA) staining.

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Stem Cell Research & Therapy

ISSN: 1757-6512