Design of mouse experimental model. (A) After being i.p. injected with CCl4 or corn oil (CTRL) twice for one week, mice were treated with NC-CM or TSG-6-CM, one time at eight days. These mice were sacrificed at 11 days. (B) CCl4-treated mice were injected with the neutralized TSG-6-CM by TSG-6 antibody or the TSG-6-CM + IgG antibody. These mice were also sacrificed at 11 days. CM, conditioned medium; CTRL, control; IgG, immunoglobulin G; i.p., intraperitoneally; NC, negative control; TSG-6, tumor necrosis factor-inducible gene 6 protein.