Effects of TSG-6 on liver histomorphology and function in CCl
-treated mice. (A) H & E staining shows the extensive cellular damage and infiltration of inflammatory cells in CCl4-treated mice with or without NC (negative control: mock transfected cell)-conditioned medium (CM). Those cellular injuries were reduced in CCl4 mice treated with TSG-6-CM (CCl4 + TSG-6). The representative images are shown at × 40 (CTRL: corn-oil-treated control mice/ CCl4:CCl4-treated mice/ CCl4 + NC: CCl4-treated mice with NC-CM). (B) Relative liver weight / body weight of mice. (C) The values of AST and ALT are graphed. (D) QRT-PCR analysis for G6pc of liver mRNA from normal (CTRL), CCl4, CCl4 with NC-CM (NC) or TSG-6-CM (TSG-6) (n ≥4 mice / group) (ANOVA, *P <0.05 versus CTRL, #P <0.05 versus CCl4, $P <0.05 versus CCl4 + NC-CM). ALT, alanine aminotransferase; ANOVA, analysis of variance; AST, aspartate aminotransferase; G6pc, glucose-6-phosphatase; TSG-6, tumor necrosis factor-inducible gene 6 protein.