TSG-6 blocks activation of hepatic stellate cells. (A) QRT-PCR analysis for tgf-β, vimentin and collagen-α1 in LX2 cells which were cultured in LX2 cell medium (LX2), NC-CM (LX2 + NC), TSG-6-CM with (LX2 + TSG-6 + Ab) or without TSG-6 antibody (LX2 + TSG-6). IgG was used as a negative control for TSG-6 antibody (LX2 + TSG-6 + IgG). Mean ± SD results are graphed. Data represent the mean ± SD of three independent experiments (ANOVA, *P <0.05 versus LX2, #P <0.05 versus NC, $P <0.05 versus TSG-6 + Ab). (B) Oil Red O staining in LX2 cells cultured in LX2, NC-CM, or TSG-6-CM (original magnification × 40). ANOVA, analysis of variance; CM conditioned medium; IgG, immunoglobulin G; NC, negative control; SD, standard deviation; TSG-6, tumor necrosis factor-inducible gene 6 protein.