Neutralization of TSG-6 by TSG-6 antibody attenuates the restoration effect of TSG-6 in liver of CCl
+ TSG-6-treated mice. (A) H & E staining shows the histomorphological changes in CCl4 + TSG-6-treated mice with (CCl4 + TSG-6 + TSG-6 antibody) (CCl4 + TSG-6 + IgG) without TSG-6 antibody (CCl4 + TSG-6) or IgG. The representative images were shown at × 40. (B) Relative liver weight/body weight of mice. (C), (D) The values of AST and ALT are graphed. (E, F, G) QRT-PCR analysis for G6pc (E), the fibrotic markers (F), tgf-β, α–sma and collagen-α1, the inflammation markers (G), tnfα, il-1β, cxcl1 and cxcl2, of liver mRNA from the treated mice (n ≥4 mice / group). Mean ± SD results are graphed (ANOVA, *P <0.05 versus TSG-6, #P <0.05 versus TSG-6 + Ab). ALT, alanine aminotransferase; ANOVA, analysis of variance; AST, aspartate aminogransferase; G6pc, glucose-6-phosphatase; IgG, immunoglobulin G; SD, standard deviation; TSG-6, tumor necrosis factor-inducible gene 6 protein.