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Figure 10 | Stem Cell Research & Therapy

Figure 10

From: Enhancement of periodontal tissue regeneration by transplantation of osteoprotegerin-engineered periodontal ligament stem cells

Figure 10

New bone regeneration assessment of hOPG-transfected PDLSCs/β-TCP constructs by circularly polarized light microscopy. (A) The osteoblasts and bone lacunas were clearly observed in the newly formed bone by using light microscopy. (B) At the same visual field, the hOPG-GFP-transfected PDLSCs were observed by using a fluorescence microscope. (C) When images (A) and (B) were overlapped, the positions of some GFP+ cells were superposed with the osteoblasts and bone lacunas. The newly formed bone was also clearly observed in Toluidine blue-stained tissue sections (D). Magnification: 100×. β-TCP, beta-tricalcium phosphate; GFP, green fluorescent protein; hOPG, human osteoprotegerin; PDLSC, periodontal ligament stem cell.

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