Figure 3

Myelin basic protein (MBP) expression. (A) The enhanced green fluorescent protein (eGFP)-positive cells were MBP-positive and located along the injection tract. (a) eGFP-positive cells (green), (b) MBP labeled with rhodamine (red), (c) nuclei were stained with 6-diamidino-2-phenylindole (DAPI) (blue), (d) merge. (B) The levels of MBP in the brain of twitcher mice were detected by Western blotting and real-time reverse transcription-polymerase chain reaction (RT-PCR). The results indicated that there was no significant difference in the level of MBP expression between the injection of oligodendrocyte progenitor cells (OPCs) and saline in twitcher mice (Western blotting: 0.478 ± 0.157 versus 0.432 ± 0.186; real-time RT-PCR: 0.81 ± 0.13 versus 0.83 ± 0.17; both P >0.05). GALC, galactocerebrosidase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.