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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Migration towards SDF-1 selects angiogenin-expressing bone marrow monocytes endowed with cardiac reparative activity in patients with previous myocardial infarction

Figure 1

In vitro migration allows for separation of viable bone marrow mononuclear cells (BM-MNCs). (A) Bar graph shows the percentage of migrated BM-MNCs, following exposure to the chemoattractant stromal cell-derived factor 1 (SDF-1) or vehicle (veh). Values are means ± standard error of the mean from 10 individual donors, with migration (migr) being performed in duplicate. **P < 0.01, versus vehmig. (B) Box plots show median values, 10th and 90th percentiles, and minimum/maximum values of trypan blue-positive BM-MNCs from the same experiment. Comparison was made between unfractioned total BM-MNCs, either freshly collected (unfractioned pre) or incubated in their original medium for the duration of migration assay (unfractioned post), and BM-MNCs that were harvested from the upper (non) and lower chamber (migr) of the migration assay following stimulation with SDF-1 or its vehicle. Trypan blue-positive BM-MNCs are fewer in the migrated fractions, indicating that spontaneous or directed migration selects for viable cells. **P < 0.001, versus unfractioned BM-MNCs, ΦΦ P < 0.01, versus the respective non-migrated fraction.

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