Figure 6

Effect of cell therapy on infarct size, interstitial fibrosis and peri-infarct vascularization. (A) Infarct size assessed at 2 weeks after myocardial infaction induction and injection of bone marrow mononuclear cells (BM-MNCs) or vehicle (VEH). (i) Representative images and (ii) bar graph showing average values. (B) Peri-infarct interstitial fibrosis. (i) Representative image and (ii) bar graph showing average values. In the representative image and insert magnification, cardiomyocytes are stained red, while the fibrotic tissue is stained blue. (C,D) Fraction of Tunel positive (C) and KI-67 positive (D) cardiomyocytes. Representative images of immunostaining (i) and average values (ii). (E-S) Myocardial vascularization assessed by counting the density of capillaries and arterioles in the LV peri-infarct zone. Representative immunohistochemistry images (E-P) and average values (Q-S). White scale bar = 20 μm. N = 7 to 10 per group. Values are means ± standard error of the mean. *P < 0.05, versus vehicle; ⁺⁺ P < 0.01, versus unfractioned; ^# P < 0.05; ^## P < 0.01, versus SDF-1^non. LV, left ventricular; SDF-1^mig, stromal cell-derived factor 1 migrated; SDF-1^non, stromal cell-derived factor 1 non-migrated.