Figure 1

Characteristics of human adipose tissue-derived stem cells from a representative donor at passage 4. (A,B) Fibroblast-like morphology of human adipose tissue-derived stem cells (hASCs) cultured in basal medium supplemented with (A) allogeneic human serum (aHS) and (B) fetal bovine serum (FBS); scale bar 500 μm. (C) Viability and proliferation of hASCs evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay performed 7, 14, 21 and 28 days after culture in both culture medium supplements. The results are based on optical density at 595 nm and represent the viability and proliferation of hASCs in basal medium supplemented with aHS and FBS. The results represent the mean ± SEM. Statistical analysis by analysis of variance and Bonferroni’s post-test was performed. *P < 0.05, versus FBS . (D-I) Multipotentiality of hASCs cultured in basal medium with aHS (left panels) and FBS (right panels). Oil red O staining showed consistent adipogenic differentiation of hASCs cultured in basal medium with (D) aHS and (E) FBS; scale bar 150 μm. von Kossa staining confirmed osteogenic differentiation of hASCs cultured in basal medium with (F) aHS and (G) FBS; scale bar 50 μm. Alcian blue staining verified chondrogenic differentiation of hASCs cultured in basal medium with (H) aHS and (I) FBS; scale bar 150 μm.