Ki-67 nuclear protein expression in human adipose tissue-derived stem cells. (A) Confocal images of the expression of Ki-67 (green) in nuclei and α-tubulin (red), showing the cell shape of human adipose tissue-derived stem cells (hASCs) cultivated in basal medium supplemented with allogeneic human serum (aHS) and fetal bovine serum (FBS) at passages 4 (P4) and 10 (P10). Cell nuclei were stained with Hoechst (blue); scale bar 20 μm. (B) Percentage of Ki-67-positive cells/total cells expressed as the mean ± SEM. Analysis of variance and Tukey’s multiple comparison test was performed. *P < 0.05, versus FBS; #
P < 0.05, versus P4.