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Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Human adipose tissue-derived stem cells cultured in xeno-free culture condition enhance c-MYC expression increasing proliferation but bypassing spontaneous cell transformation

Figure 3

Ki-67 nuclear protein expression in human adipose tissue-derived stem cells. (A) Confocal images of the expression of Ki-67 (green) in nuclei and α-tubulin (red), showing the cell shape of human adipose tissue-derived stem cells (hASCs) cultivated in basal medium supplemented with allogeneic human serum (aHS) and fetal bovine serum (FBS) at passages 4 (P4) and 10 (P10). Cell nuclei were stained with Hoechst (blue); scale bar 20 μm. (B) Percentage of Ki-67-positive cells/total cells expressed as the mean ± SEM. Analysis of variance and Tukey’s multiple comparison test was performed. *P < 0.05, versus FBS; # P < 0.05, versus P4.

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