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Figure 4 | Stem Cell Research & Therapy

Figure 4

From: Human adipose tissue-derived stem cells cultured in xeno-free culture condition enhance c-MYC expression increasing proliferation but bypassing spontaneous cell transformation

Figure 4

Proliferation kinetics of human adipose tissue-derived stem cells cultured in basal medium supplemented with allogeneic human serum and fetal bovine serum. (A) Mean cumulative population doublings measured and expressed as a function of passage number for allogeneic human serum (aHS; black bars) and fetal bovine serum (FBS; white bars). The results represent the mean ± SEM. Analaysis of variance (ANOVA) with Bonferroni's post-test was performed. P < 0.05, comparing the condition of basal medium with aHS to basal medium with FBS at all passages and for comparisons between all passages for the condition of basal medium supplemented with aHS. For comparisons between all passages for the condition of basal medium supplemented with FBS: *P < 0.05 for the P1 × P3 comparison; # P < 0.05 for the P3 × P5 comparison; + P < 0.05 for the P5 × P6 comparison; ɸ P < 0.05 for the P6 × P8 comparison. (B) Growth curve assessing the proliferation kinetics of human adipose tissue-derived stem cells cultivated in basal medium supplemented with aHS and FBS at days 3, 5, 9, and 11. ANOVA with Bonferroni's post-test was performed. *P < 0.05, comparing hASCs cultured in basal medium with aHS to FBS at days 7, 9 and 11; # P < 0.05, comparing the growth between days 7 and 9 in basal medium supplemented with aHS; ɸ P < 0.05, for the comparison between days 9 and 11 in basal medium supplemented with FBS.

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