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Figure 5 | Stem Cell Research & Therapy

Figure 5

From: Human adipose tissue-derived stem cells cultured in xeno-free culture condition enhance c-MYC expression increasing proliferation but bypassing spontaneous cell transformation

Figure 5

Transcription factors expression of human adipose tissue-derived stem cells cultured in basal medium supplemented with allogeneic human serum and fetal bovine serum. (A) Representative immunoblot of c-FOS transcription factor (60 kDa) expression from nuclear fractions of human adipose tissue-derived stem cells (hASCs) at passage 4. Densitometric analyses showed no significant difference in the c-FOS expression in hASCs in both culture medium supplements. Lamin B (60 kDa) was used as a purity control for the nuclear fractions (n, nuclear; nn, non-nuclear). The results were plotted and Student’s t‐test was performed. No significant differences were detected (statistical significance was set at P < 0.05, two‐tailed). (B) Representative immunoblot for c-MYC transcription factor (120 kDa) expression in total extracts of hASCs at passages 4 (P4) and 10 (P10). Densitometric analyses showed that c-MYC in hASCs cultured in basal medium supplemented with allogeneic human serum (aHS) is expressed more than in fetal bovine serum (FBS) in both passages evaluated. GAPDH (37 kDa) was used as a purity control for the total extracts. Bar graph summary showing that hASCs cultured in basal medium with aHS had increased expression of c-MYC compared to cells cultured in basal medium supplemented with FBS. Analysis of variance and Tukey's multiple comparison test was performed. *P < 0.05.

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