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Figure 6 | Stem Cell Research & Therapy

Figure 6

From: Human adipose tissue-derived stem cells cultured in xeno-free culture condition enhance c-MYC expression increasing proliferation but bypassing spontaneous cell transformation

Figure 6

Gene expression of human adipose tissue-derived stem cells cultured in basal medium supplemented with allogeneic human serum and fetal bovine serum. Real-time quantitative PCR analyses showing the expression of (A) MYC, (B) TERT (C) CDKN2A and (D) ERBB2 in hASCs at passages 4 (P4) and 10 (P10). The gene expression was calculated by the 2-ΔCt method and GAPDH was used to normalize the data. Analysis of variance and Tukey’s multiple comparison test were performed (n = 3). *P < 0.05, comparing the gene expression of human adipose tissue-derived stem cells (hASCs) cultivated in allogeneic human serum (aHS) versus fetal bovine serum (FBS) at passage 4; # P < 0.05, comparing the gene expression of hASCs cultivated in aHS at passage 4 and passage 10; Ψ P < 0.05, comparing the gene expression of hASCs cultivated in FBS at passage 4 and passage 10; Δ P < 0.05, comparing the gene expression of hASCs cultivated in aHS versus FBS at passage 10.

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