Senescence-associated β-galactosidase expression of human adipose tissue-derived stem cells. (A-B) Single cell senescence-associated β-galactosidase (SA-β-gal) staining showing the formation of a blue precipitate in senescent cells (scale bar 50 μm). Representative images of human adipose tissue-derived stem cells (hASCs) cultivated in (C) allogeneic human serum (aHS) and (D) fetal bovine serum (FBS) at passage 10 showing earlier β-gal expression by hASCs cultivated in medium with FBS (scale bar 100 μm). (E) Percentage of β-gal-positive cells/total cells at each passage evaluated, expressed as the mean ± SEM. Analysis of variance with Bonferroni’s post-test was performed. *P < 0.05, comparing the condition of basal medium with aHS to basal medium with FBS at each passage after passage 8. #
P < 0.05, indicates the passage at which the percentage of senescent cells in the same culture condition were significantly increased.