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Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Large-scale expansion of Wharton’s jelly-derived mesenchymal stem cells on gelatin microbeads, with retention of self-renewal and multipotency characteristics and the capacity for enhancing skin wound healing

Figure 3

Cell cycle and EdU assay of hWJ-MSC. Merged image of EdU staining (green) and Hoechst 33342 staining (blue) for 2D and 3D cultured cells (A), bar = 100 μm. Flow cytometry with propidium iodide staining for 2D cultures and 3D cultures (B). A histogram comparing the percentage of EdU-positive nuclei in 2D and 3D cultured cells.*P <0.05 (2D cultured cells versus 3D cultured cells, at 100 cells/cm2), *P <0.05 (3D cultured cells versus 2D cultured cells at 1,000 cells/cm2) (C). A histogram (D) comparing the cell proliferation index of 2D and 3D cultures. *P <0.05 (2D cultured cells versus 3D cultured cells at 100 cells/cm2), *P <0.05 (3D cultured cells versus 2D cultured cells at 1,000 cells/cm2). EdU, ethynl-2′-deoxyuridine; hWJ-MSC, human Wharton’s jelly-derived mesenchymal stem cells; 2D, plate cultures; 3D, spinning bottle cultures.

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