Surface marker profiles of hWJ-MSC after 2D and 3D culturing. hWJ-MSC collected from tissue culture plates (2D) or microbeads (3D) were stained with the indicated primary antibodies against CD mesenchymal markers. Alexa Fluor 488- or Alexa Fluor 647-R-phycoerythrin-conjugated secondary antibody was used to detect the primary antibodies. Hoechst 33342 was used to track the nucleus. Immunofluorescence (A) and flow cytometry (B) results showed that the two types of cultured cells all express the mesenchymal markers CD90, CD105, CD73, and CD44, but did not express CD31 or CD45. Statistical analysis showed no differences in the expression levels of these markers (P >0.01) (C). hWJ-MSC, human Wharton’s jelly-derived mesenchymal stem cells.