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Figure 6 | Stem Cell Research & Therapy

Figure 6

From: Large-scale expansion of Wharton’s jelly-derived mesenchymal stem cells on gelatin microbeads, with retention of self-renewal and multipotency characteristics and the capacity for enhancing skin wound healing

Figure 6

Multipotency of hWJ-MSC as assessed by chemical staining and qRT-PCR. hWJ-MSC were collected from tissue culture plates (2D) or microbeads (3D), and cultured in osteogenic, adipogenic, and chondrogenic medium. Oil red O, alizarin red, and toluidine blue were used to evaluate osteogenesis, adipogenesis, and chondrogenesis, respectively; the control groups are shown in (A). A histogram (B, C) showing the absence of significant differences between 2D cultured cells and 3D cultured cells after staining with oil red O and alizarin red S. RT-PCR showed that the 2D and 3D cultured cells all expressed AP2 (D), RUNX2 (E), and COLII (F), and the qRT-PCR data indicated that the mRNA levels of AP2 (G), RUNX2 (H), and COLII (I) were not statistically significantly different between 2D cultured cells and 3D culture cells (P >0.01). hWJ-MSC, human Wharton’s jelly-derived mesenchymal stem cells.

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Stem Cell Research & Therapy

ISSN: 1757-6512