Figure 3
From: Nuclear reprogramming with a non-integrating human RNA virus
Expression of pluripotency-associated markers in measles virus (MV)-derived induced pluripotent stem cell (iPSC)-like clones. Three MV- and one lentiviral vector (LV)-derived iPSC clone (#1, #2, #3, and 4LV) were cultured under feeder-free conditions on a Matrigel-based slide and examined for expression of human pluripotent stem cell markers by immunofluorescence (A) or alkaline phosphatase (B). Both passages 6 and 15 were tested. Scale bars: 50 μm. (C) Reverse transcription-polymerase chain reaction analysis assessing transcription of key pluripotency-associated markers (OCT4, SOX2, KLF4, NANOG, GDF3, hTERT, and cMYC) using total cellular RNA of the same four iPSC clones at passages 6 and 15. GAPDH (glyceraldehyde 3-phosphate dehydrogenase) is the cellular internal control, and water is the negative control. (D) G-banding chromosome analysis of iPSC clone #3.