Figure 5
From: Nuclear reprogramming with a non-integrating human RNA virus
Cardiac differentiation of measles virus (MV)-derived induced pluripotent stem cell (iPSC)-like clones. Three MV- and one lentiviral vector (LV)-derived iPSC clone (#1, #2, #3, and 4LV) were analyzed for cardiac differentiation. Immunofluorescence for cardiac markers is shown. iPSCs before (−) and after (+) cardiac differentiation were fixed, permeabilized, and stained with antibody to α-actinin (green) or troponin (green). Nuclei were counterstained by 4′,6-diamidino-2-phenylindole (DAPI) (blue). Scale bars: 50 μm.