Detection of injected cells in pig femoral head (FH) 30 minutes after human bone marrow mesenchymal stromal cell (hBMSCs) injection. (a-e) Masson’s Tri-chrome staining. (a, b) Normal pig FH sections. (c-e) Injected pig FH sections. Arrows indicate the hBMSC area. (e) Localization of sections (c), (d), (h), and (i). (f-i)
In situ hybridization of human Alu sequences (Alu-ISH). (f) Negative control tissue of pig FH injected with human cells in omitting Alu probe stained with the hematoxylin only. (g) Negative control tissue of pig FH injected with physiological saline without human cells, with Alu probe and hematoxylin counterstaining. (h, i) Injected pig FH with human cells detected by Alu-probe staining and counterstained with the hematoxylin. The positive nuclei for Alu-probe staining appear in dark brown (arrows). Magnifications: 10× (a, c, f-h), 20× (b, d), 1× (e), and 40× (i).