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Figure 5 | Stem Cell Research & Therapy

Figure 5

From: Development of a simple procedure for the treatment of femoral head osteonecrosis with intra-osseous injection of bone marrow mesenchymal stromal cells: study of their biodistribution in the early time points after injection

Figure 5

Histological analysis of in vivo bone formation after 7 weeks of ectopic implantation into immunodeficient mice. Scaffolds (a, b) without pig bone marrow mesenchymal stromal cells (pBMSCs) (a) Magnifications: 4×, (b) Magnifications: 20×. (c, d) with 300,000 pBMSCs directly loaded onto the bone scaffold during the surgery. Cells from three different pigs were tested. (c) Magnifications: 4×, (d) Magnifications: 20×. Decalcified implants (n = 6 per condition) were embedded in paraffin and stained with Masson’s Tri-chrome (blue/green = collagen and non-mineralized bone; red = mineralized bone scaffold; purple = nuclei; and pink = cytoplasm). Dotted lines correspond to the areas of new bone formation. NB, new bone; Sc, scaffold ; FT, fibrous tissue.

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