Table 1 Tracking of injected human bone marrow mesenchymal stromal cells in vivo by molecular biology: detection by quantitative real-time polymerase chain reaction of the human RNase P gene
Samples | Delay after injection | RNase detection | ||
|---|---|---|---|---|
Pig negative control | Organs | Lung | No injection | No detection |
Liver | ||||
Spleen | ||||
Kidney | ||||
Blood | ||||
Bone marrow | ||||
Pig sample after hBMSCs injection | Adjacent tissues of FH n = 6 samples per tissue | Capsule | 30 minutes | No detection |
Periarticular muscles | ||||
Gluteus maximus muscle | ||||
Round ligament | ||||
Organs n = 6 samples per organ | Lung | 30 minutes and 24 hours | No detection | |
Liver | ||||
Spleen | ||||
Kidney | ||||
Blood n = 4 samples per condition | 0 | No detection | ||
1 minute | ||||
5 minutes | ||||
15 minutes | ||||
30 minutes | ||||
1 hour | ||||
24 hours | ||||
Bone marrow n = 4 samples per condition | 0 | No detection | ||
24 hours | ||||