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Figure 4 | Stem Cell Research & Therapy

Figure 4

From: Microencapsulated equine mesenchymal stromal cells promote cutaneous wound healing in vitro

Figure 4

Mesenchymal stromal cells (MSCs) retain stem cell characteristics after microencapsulation (n = 3). (A) Expression of surface markers, as detected by flow cytometry, on MSCs removed from capsules after 2 days post-encapsulation as compared with control MSCs that were never encapsulated. Percentage of total cells positive is shown on the left, and representative histograms are shown on the right. *P <0.05. (B) Representative images of MSCs stained with Alizarin Red, Alcian Blue, and Oil Red O to detect osteocyte, chondrocyte, and adipocyte differentiation, respectively. Left column: undifferentiated MSCs cultured in expansion medium; center column: control MSCs that were never encapsulated cultured in MSC differentiation media; and right column: MSCs removed from capsules after 2 days post-encapsulation cultured in MSC differentiation media. Scale bars = 10 μm. (C) Population doubling times of MSCs removed from capsules after 2 days post-encapsulation as compared with control MSCs that were never encapsulated. MHC, major histocompatibility complex.

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