Figure 5
Effect of bone marrow mesenchymal stem cells on myeloid-derived suppressor cells in vitro ( n  = 6). Bone marrow cells (5 × 105 cells) obtained from femurs and tibias of C57 and BALb/c mice were cultured in RPMI 1640 medium, alone, or in the presence of 10%, 30%, or 60% v/v tumor supernatant culture medium (ST), or co-cultured with 1 × 105 syngeneic bone marrow mesenchymal stem cells (BMSCs). After 24, 48, and 72 hours, floating cells were gently collected and numbered. The absolute number of Gr-1+CD11b+ myeloid-derived suppressor cells (MDSCs) was calculated as described in Materials and methods. (A) Effect of BMSCs on MDSCs in the presence of B16-F10 culture medium. (B) Effect of BMSCs on MDSCs in the presence of H22 culture medium. (C) BMSC/bone marrow co-culture in the presence of B16-F10 culture medium. (D) BMSC/bone marrow co-culture in the presence of H22 culture medium. # P <0.05; § P <0.01 compared with the control (CT) group.