Figure 6
Effect of bone marrow mesenchymal stem cells on CD3 + IFNγ + T cells in vitro ( n  = 6). Peripheral blood (PB) white cells (5 × 105 cells) obtained from C57 and BALb/c mice were co-cultured with 1 × 105 NIH-3T3 or syngeneic BMSCs in 60% v/v tumor supernatant culture medium with or without the presence of 1 × 105 bone marrow suspension cells obtained from femurs and tibias of C57 and BALb/c mice. After 24, 48, and 72 hours, floating cells were gently collected and numerated. The percentage of CD3+IFNγ+ T cells in suspension cells was analyzed as described in Materials and methods. (A) Effect of BMSCs and MDSCs on PB white CD3+IFNγ+ T cells in the presence of B16-F10 culture medium. (B) Effect of BMSCs and MDSCs on PB white CD3+IFNγ+ T cells in the presence of H22 culture medium. # P <0.05. +BMSCs, PB white cells + MDSCs + BMSCs; CT, PB white cells + MDSCs; IFNγ, interferon gamma; +NIH3T3, PB blood white cells + MDSCs + NIH-3T3.