Figure 4

Localization of ARPC2 and actin in neural stem cell-derived oligodendrocyte precursor cells (NSC-OPCs). (A) Migration of ARPC2^+/+ NSC-OPCs out of oligosphere. (B) Migration of ARPC2^−/− NSC-OPCs out of oligosphere. (A, B) Cells labeled with anti-platelet-derived growth factor receptor alpha (anti-PDGFRα) antibody (red). (A′, B′) Magnified images of inset indicated in (A and B), respectively Scale bar: 100 μm. (C) Differentiation of ARPC2^+/+ NSC-OPCs to oligodendrocytes. (D) Differentiation of ARPC2^+/+ NSC-OPCs to oligodendrocytes. Scale bar: 50 μm. (E-G) ARPC2^+/+ NSC-OPCs are labeled with anti-ARPC2 antibody. Arrows show labeled ARPC2 at leading edges of the cells. (H-J) ARPC2 is absent at leading edge of ARPC2^−/− NSC-OPCs. (E, H) Cells labeled with anti-ARPC2 antibody (green). (F, I) Cells labeled with Alexa Fluor 568 phalloidin (red). Scale bar: 30 μm.