Figure 8From: Human umbilical cord blood-derived mononuclear cells improve murine ventricular function upon intramyocardial delivery in right ventricular chronic pressure overloadMononuclear cells of UCB paracrine action and vascular reactivity in right ventricle. Immunofluorescence analysis for the angiogenesis marker CD31 was performed on paraffin embedded cell-treated and PAB only heart tissue. (A-F) Vascularity in RV and LV was assessed, and resulted in an increase in the number of blood vessels in RV after four weeks of cells delivery (P = 0.01 versus PAB, scale bars = 100 μm). (G, H) CD31 positive vessels (endothelial-red) were analyzed through CellSens Dimension software (n = 4) and non-specific/background was filtered out for final calculation. A representative image (LV, RV) of immunofluorescence staining is illustrated. LV, left ventricle; PAB, pulmonary artery banding; RV, right ventricle; UCB, unbilical cord blood.Back to article page