Figure 4
From: Acyclic retinoid induces differentiation and apoptosis of murine hepatic stem cells
Acyclic retinoid induces cellular apoptosis of hepatic stem cells. (A) Observation of the formation of vacuolar cells after 24 hours of incubation with acyclic retinoid (ACR), and the ratio of vacuolar cells (n = 9). (B) Immunofluorescence for Caspase 3 after incubation with ACR, and the ratio of Caspase 3-positive cells (n = 6). (C) Flow cytometry analysis of Annexin V-positive cells after incubation with ACR (n = 3). (D) Real-time PCR analysis of the relative mRNA expression of Annexin V and Caspase 3 after incubation with ACR (n = 5–8). Scale bar = 200 μm. Data are shown as means ± SD. Mann–Whitney U-test. *P < 0.05; **P < 0.01.