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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Predictions for optimal mitigation of paracrine inhibitory signalling in haemopoietic stem cell cultures

Figure 1

Mitigation strategies for minimising the effects of paracrine inhibitory signalling. Mixing: cell culture medium is drawn up and down repeatedly. The mixing frequency f mix determines how often mixing occurs. The volume of the culture remains constant. Media-exchange: a fraction α of the total cell culture volume is replaced by fresh media after every time interval 1/f ME, where f ME is the media-exchange frequency. Again, the volume of the culture remains constant. Fed-batch: fresh medium is continuously added to the culture at a constant rate of Q ml/day. Medium is not removed from the culture, and hence the culture volume increases over time. Perfusion: fresh medium is continually added to the culture system, and spent medium is continually removed from the culture system, both at a constant rate of Q ml/day. The cell culture volume remains constant over time.

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