Observation of green fluorescent protein-positive (GFP+) bone marrow cells (BMCs) migrating to tissue surrounding a wound, and to adipose tissue-derived stromal cell (ASC) and bone marrow-derived stromal cell (ST2) transplantation area. (A) Bright field images of an ear in which was punched a hole (left two panels) and fluorescence images with a fluorescent filter for GFP (right two panels) at 5 hours after perforation. GFP+ BMCs migrated to tissue surrounding the hole (right panel). (B) Temporal changes in migration of GFP+ BMCs and sizes of holes. (C) Fluorescence images were taken using a fluorescent filter for GFP at 6 hours and at 3 days after intradermal transplantation of ASCs into mouse ears. GFP+ BMCs gradually migrated towards ASC transplantation area; arrows show the migrated GFP+ BMCs. (D,E) Fluorescence images of GFP+ BMC migration at 1 and 7 days after transplantation of PKH26 (red)-labeled ASCs (D) and PKH26-labeled ST2 cells (E); images were taken using fluorescent filters for red fluorescent protein and GFP. (F) Fluorescence images at 1 and 7 days after saline injection. All scale bars = 2 mm.