Figure 2From: Ascorbic acid improves pluripotency of human parthenogenetic embryonic stem cells through modifying imprinted gene expression in the Dlk1-Dio3 regionIdentification of human parthenogenetic embryonic stem cells. (A) Colony of human parthenogenetic embryonic stem cells; (B) positive staining for alkaline phosphatase; (C) normal 46, XX karyotype at passage 20; (D) positive staining for OCT4; (E) positive staining for NANOG; (F) positive staining for TRA-1-60; (D1-F1) nuclear staining with Hoechst 33342; (D2-F2) merged images for OCT4, NANOG and TRA-1-60. Bar is 100 μm.Back to article page