Figure 4

MSCs suppressed the expression of inflammatory cytokines. (A) Bio-Plex Assay was used to analyze cytokine changes in mice plasma, including IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-12, IL-13, G-CSF and RANTES. (B) The expression levels of colonic inflammatory cytokines (IL-1β, IL-6 and TNF-α) from different groups were evaluated by Q-PCR. (C) The expression of p-P65 examined by immunohistochemistry staining comparing the colons in the treated and untreated groups. Values are expressed as means ± SEM. *P <0.05, or **P <0.01. MSCs, mesenchymal stem cells; SEM, standard error of the mean.