Western blot analysis of protein expression. Western blot analysis of proteins reflects the results observed in the microarray and RT q-PCR gene experiments. Note that HMGA2 protein is low or non-existent in permanent pulp cells. Quantification on the intensity levels of the Western blot bands was performed, B-E. A) Western blot bands, B) HMGA2, C) CDC2A, D) FABE, E) SERPINF1. Dec: Pulp cells from deciduous teeth, n = 3 Perm: Pulp cells from permanent teeth, n = 3. *indicates P ≤ 0.05, unpaired Student’s t-test.