Mitochondrial potential analysis in induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) and neonatal cardiomyocytes (N-CMs). (A) Representative images of tetramethylrhodamine methyl ester (TMRM) staining of iPS-CMs and N-CMs after 3 hours hypoxia and glucose/serum deprivation (GSD) compared to normoxia control (21% O2, 15% fetal bovine serum and high glucose). Scale bar 100 μm. (B) Quantification of TMRM fluorescence intensity of cells cultured in normoxia versus cells cultured in hypoxia and GSD (#
P < 0.001, versus normoxia control; ##
P < 0.001, iPS-CMs versus N-CMs in normoxia).