Figure 11
Mitochondrial potential analysis in induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) and neonatal cardiomyocytes (N-CMs). (A) Representative images of JC-1 staining of iPS-CMs and N-CMs after 3 hours hypoxia and glucose/serum deprivation (GSD) compared to normoxia control (21% O2, 15% fetal bovine serum and high glucose) (# P < 0.01, versus normoxia control). Scale bar 100 μm. Note the nuclear shrinking in cells with diffuse JC-1 staining. (B) Proportion of cells with diffuse JC-1 staining expressed as % of cells versus normoxia/full medium (# P ≤ 0.01). GFP, green fluorescent protein; JC-1, 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazol-carbocyanine iodide.