Effect of hypoxia and nutrient deprivation on metabolic activity of induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) and neonatal cardiomyocytes (N-CMs). (A) Activity of cellular oxidoreductases in iPS-CMs and N-CMs determined by MTS assay, normalized to normoxia/full medium data. *P ≤ 0.05, for iPS-CMs versus normoxia (Bonferroni test); #
P ≤ 0.05, versus different hypoxia and glucose/serum deprivation (GSD) durations (Bonferroni test); ##
P < 0.001, iPS-CMs versus N-CMs by analysis of variance. (B) ATP content in iPS-CMs and N-CMs determined by CellTiter Glo assay. *P ≤ 0.05, versus normoxia (0 hours, Bonferroni test); #
P ≤ 0.05, versus N-CMs. (C) Average number of iPS-CMs and N-CMs per well.