Figure 9

Response of murine induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) and neonatal cardiomyocytes (N-CMs) to in vitro hypoxia and nutrient deprivation. (A) Representative photomicrographs (scale bar 100 μm) and (B) quantitative analysis of poly-caspase-positive, 7-aminoactinomycin D (7-AAD)-positive iPS-CMs and N-CMs after 3 hours incubation in hypoxia and glucose/serum deprivation (GSD) (^# P ≤ 0.05, versus normoxia/full medium). (C) Caspase-8, -9, and 3/7 activities in iPS-CMs and N-CMs after 3 hours incubation in hypoxia/GSD. Data are normalized to those normoxia controls. *P ≤ 0.05, versus normoxia/full medium; ^# P ≤ 0.05, iPS-CMs versus N-CMs in hypoxia/GSD. (D) Increase in TUNEL-positive cells after 3 hours incubation in hypoxia/GSD (^# P = 0.001). FBS, fetal bovine serum.