Susceptibility of murine induced pluripotent stem cell-derived cardiomyocytes to hypoxia and nutrient deprivation

Andreja Brodarac; Tomo Šarić; Barbara Oberwallner; Shokoufeh Mahmoodzadeh; Klaus Neef; Julie Albrecht; Karsten Burkert; Matteo Oliverio; Filomain Nguemo; Yeong-Hoon Choi; Wolfram F Neiss; Ingo Morano; Jürgen Hescheler; Christof Stamm

doi:10.1186/s13287-015-0057-6

Stem Cell Research & Therapy

Table 1 Primers used for real-time quantitative PCR

From: Susceptibility of murine induced pluripotent stem cell-derived cardiomyocytes to hypoxia and nutrient deprivation

Gene	Primer sequence (5′→3′)	Annealing temperature (°C)	Reference
Hif 1α	Fw: CATGATGGCTCCCTTTTTCA	63	[55]
Hif 1α	Rev: GTCACCTGGTTGCTGCAATA	63	[55]
Hif 2α	Fw: GGGAACACTACACCCAGTGC	63	[55]
Hif 2α	Rev: TCTTCAAGGGATTCTCCAAGG	63	[55]
Vegf	Fw: ACTGGACCCTGGCTTTACTG	63	[56]
Vegf	Rev: TCACTTCATGGGACTTCTGC	63	[56]
Glut-1	Fw: GCTTCCTGCTCATCAATCGT	63	[56]
Glut-1	Rev: CTTCTTCTCCCGCATCATCT	63	[56]
HSP70	Fw: TGGTGCTGACGAAGATGAAG	63	[57]
HSP70	Rev: AGGTCGAAGATGAGCACGTT	63	[57]
PKCδ	Fw: CAGACCAAGGACCACCTGTT	63	[58]
PKCδ	Rev: GCATAAAACGTAGCCCGGTA	63	[58]
PKCε	Fw: GAGGACTGGATTGACCTGGA	63	[58]
PKCε	Rev: ATCTCTGCAGTGGGAGCAGT	63	[58]
PKCθ	Fw: ATGGACAACCCCTTCTACCC	63	[58]
PKCθ	Rev: GCGGATGTCTCCTCTCACTC	63	[58]
Bcl2	Fw: TGTGTGTGGAGAGCGTCAACA	65	[59]
Bcl2	Rev: TGCCGGTTCAGGTACTCAGTC	65	[59]
Bax	Fw: GCGTGGTTGCCCTCTTCTACTTTG	65	[60]
Bax	Rev: AGTCCAGTGTCCAGCCCATGATG	65	[60]
Bad	Fw: GGGAGCAACATTCATCAGCAGG	65	[60]
Bad	Rev: CATCCCTTCATCCTCCTCGGTC	65	[60]
Bcl-xL	Fw: AACATCCCAGCTTCACATAACCCC	65	[60]
Bcl-xL	Rev: GCGACCCCAGTTTACTCCATCC	65	[60]
P53	Fw: ATTTGTATCCCGAGTATCTG	63	[56]
P53	Rev: GGTATACTCAGAGCCGGCCT	63	[56]
Fas	Fw: AGGACTGCAAAATGAATGGG	60	[61]
Fas	Rev: GGGTGCAGTTTGTTTCCACT	60	[61]
FADD	Fw: GGGGACTCATCCTGTTTTCT	60	[62]
FADD	Rev: ATGCATAGTCTGGGGAGTCA	60	[62]
Gata4	Fw: AATGCCTGTGGCCTCTATCA	60	[63]
Gata4	Rev: GGTCTCGCTCCTGGAAGATG	60	[63]
Mef2c	Fw: ATTTGGGAACTGAGCTGTGC	60	[63]
Mef2c	Rev: CGCTCATCCATTATCCTCGT	60	[63]
Myh6	Fw: CCAACACCAACCTGTCCAAGT	60	[64]
Myh6	Rev: AGAGGTTATTCCTCGTCGTGCAT	60	[64]
Nkx2.5	Fw: CCAGAACCGTCGCTACAAGT	60	[65]
Nkx2.5	Rev: GGGTAGGCGTTGTAGCCATA	60	[65]
Tnnt2	Fw: GCCAAAGATGCTGAAGAAGG	60	[66]
Tnnt2	Rev: TTCTCGAAGTGAGCCTCGAT	60	[66]
Gapdh	Fw: GGGGACTCATCCTGTTTTCT	60	[64]
Gapdh	Rev: ATGCATAGTCTGGGGAGTCA	60	[64]

Fw, forward; Rev, reverse.

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