Figure 2

IL-1β induced the production of inflammation-related factors by TFs. (A) IL-1β-induced up-regulation of inflammation-related genes by TFs occurs in a dose- and time-dependent manner. Gene expression for each dosage is normalized to control (0 ng/ml) (* P <0.05, multi-factor ANOVA, N = 4, except for IL-1β on day 1, N = 3). (B) IL-1β (10 ng/ml) induced MMP3 and IL-1β protein expression by TF (und = undetected, * P <0.05, ANOVA, significant effect of IL-1β treatment and time, N = 4). (C) IL-1β (10 ng/ml) led to decreased TF viability (* P <0.05, two-way ANOVA, significant effect of IL-1β treatment, N = 3). ANOVA, analysis of variance; MMP3, matrix metalloproteinase 3; TF, tendon fibroblasts.