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Table 2 ASCs suppressed the effects of M1 and M0 macrophages on TF secretion of pro-inflammatory factors

From: Adipose-derived mesenchymal stromal cells modulate tendon fibroblast responses to macrophage-induced inflammation in vitro

Day 1 M0 versus M0 + ASCs M1 versus M1 + ASCs
  Mean P Value Mean P Value
TNF 1.8 ± 1.6 0.620 −1.7 ± 0.3 0.060
IL-1b 3.4 ± 3.4 0.340 1.0 ± 0.6 0.510
COX2 −1.2 ± 0.2 0.230 −1.1 ± 0.3 0.440
MMP1a −1.9 ± 0.2 0.020 −1.9 ± 0.3 0.060
MMP3 −1.9 ± 0.2 0.010 −1.1 ± 0.2 0.350
MMP13 −1.3 ± 0.6 0.220 −1.5 ± 0.3 0.150
COL1 1.3 ± 0.3 0.030 1.1 ± 0.3 0.660
COL3 1.3 ± 0.5 0.280 −1.1 ± 0.2 0.440
BGN 1.1 ± 0.1 0.250 −1.1 ± 0.3 0.520
DCN 1.2 ± 0.4 0.440 −1.2 ± 0.3 0.240
SCX −1.8 ± 0.1 <0.001 −1.1 ± 0.1 0.100
TNMD −1.5 ± 0.2 0.030 1.0 ± 0.1 0.580
Day 5 M0 versus M0 + ASCs M1 versus M1 + ASCs
  Mean P Value Mean P Value
TNF 2.7 ± 1.9 0.160 −1.9 ± 0.3 0.140
IL-1b 3.7 ± 3.3 0.180 −1.8 ± 0.6 0.280
COX2 −1.0 ± 0.1 0.760 −10 ± 0.0 <0.001
MMP1a −1.5 ± 0.3 0.240 −7.7 ± 0.1 0.060
MMP3 1.5 ± 0.2 0.050 −4.6 ± 0.2 0.050
MMP13 1.9 ± 0.4 0.040 5.0 ± 6.6 0.490
COL1 1.0 ± 0.1 0.940 1.8 ± 0.1 <0.001
COL3 1.4 ± 0.3 0.290 2.0 ± 0.8 0.140
BGN 1.3 ± 0.3 0.390 −1.2 ± 0.2 0.300
DCN 1.2 ± 0.1 0.280 1.0 ± 1.1 0.770
SCX 1.2 ± 0.1 0.150 −1.8 ± 0.1 0.030
TNMD 1.4 ± 0.1 0.100 1.2 ± 0.3 0.430
  1. ASCs had the greatest effect on M1 macrophages on day 5. Gene expression of inflammation-, degradation-, matrix production-, and tendon differentiation-related factors after one day (top) and five days (bottom) of co-culture with macrophages (M0, M1) or tri-culture with macrophages and ASCs (M0 + ASC, M1 + ASC). Data from each group were normalized first to untreated TFs and then to their paired control (that is, (M0 + ASC)/M0, (M1 + ASC)/M1). Data are presented as mean ± SD; N = 5 for day 1, N = 2 to 3 for day 5. This data is presented graphically in the supplemental document, Additional file 1: Figure S6. ASCs, adipose-derived mesenchymal stromal cells; biglycan; COL, collagen; COX, cyclooxygenase; DCN, decorin; IL-1β, interleukin-1β; MMP, matrix metalloproteinase; SCX, scleraxis; TFs, tendon fibroblasts; TNF, tumor necrosis factor; TNMD, tenomodulin.