|
Day 1
|
M0 versus M0 + ASCs
|
M1 versus M1 + ASCs
|
| |
Mean
|
P
Value
|
Mean
|
P
Value
|
|
TNF
|
1.8 ± 1.6
|
0.620
|
−1.7 ± 0.3
|
0.060
|
|
IL-1b
|
3.4 ± 3.4
|
0.340
|
1.0 ± 0.6
|
0.510
|
|
COX2
|
−1.2 ± 0.2
|
0.230
|
−1.1 ± 0.3
|
0.440
|
|
MMP1a
|
−1.9 ± 0.2
|
0.020
|
−1.9 ± 0.3
|
0.060
|
|
MMP3
|
−1.9 ± 0.2
|
0.010
|
−1.1 ± 0.2
|
0.350
|
|
MMP13
|
−1.3 ± 0.6
|
0.220
|
−1.5 ± 0.3
|
0.150
|
|
COL1
|
1.3 ± 0.3
|
0.030
|
1.1 ± 0.3
|
0.660
|
|
COL3
|
1.3 ± 0.5
|
0.280
|
−1.1 ± 0.2
|
0.440
|
|
BGN
|
1.1 ± 0.1
|
0.250
|
−1.1 ± 0.3
|
0.520
|
|
DCN
|
1.2 ± 0.4
|
0.440
|
−1.2 ± 0.3
|
0.240
|
|
SCX
|
−1.8 ± 0.1
|
<0.001
|
−1.1 ± 0.1
|
0.100
|
|
TNMD
|
−1.5 ± 0.2
|
0.030
|
1.0 ± 0.1
|
0.580
|
|
Day 5
|
M0 versus M0 + ASCs
|
M1 versus M1 + ASCs
|
| |
Mean
|
P
Value
|
Mean
|
P
Value
|
|
TNF
|
2.7 ± 1.9
|
0.160
|
−1.9 ± 0.3
|
0.140
|
|
IL-1b
|
3.7 ± 3.3
|
0.180
|
−1.8 ± 0.6
|
0.280
|
|
COX2
|
−1.0 ± 0.1
|
0.760
|
−10 ± 0.0
|
<0.001
|
|
MMP1a
|
−1.5 ± 0.3
|
0.240
|
−7.7 ± 0.1
|
0.060
|
|
MMP3
|
1.5 ± 0.2
|
0.050
|
−4.6 ± 0.2
|
0.050
|
|
MMP13
|
1.9 ± 0.4
|
0.040
|
5.0 ± 6.6
|
0.490
|
|
COL1
|
1.0 ± 0.1
|
0.940
|
1.8 ± 0.1
|
<0.001
|
|
COL3
|
1.4 ± 0.3
|
0.290
|
2.0 ± 0.8
|
0.140
|
|
BGN
|
1.3 ± 0.3
|
0.390
|
−1.2 ± 0.2
|
0.300
|
|
DCN
|
1.2 ± 0.1
|
0.280
|
1.0 ± 1.1
|
0.770
|
|
SCX
|
1.2 ± 0.1
|
0.150
|
−1.8 ± 0.1
|
0.030
|
|
TNMD
|
1.4 ± 0.1
|
0.100
|
1.2 ± 0.3
|
0.430
|
- ASCs had the greatest effect on M1 macrophages on day 5. Gene expression of inflammation-, degradation-, matrix production-, and tendon differentiation-related factors after one day (top) and five days (bottom) of co-culture with macrophages (M0, M1) or tri-culture with macrophages and ASCs (M0 + ASC, M1 + ASC). Data from each group were normalized first to untreated TFs and then to their paired control (that is, (M0 + ASC)/M0, (M1 + ASC)/M1). Data are presented as mean ± SD; N = 5 for day 1, N = 2 to 3 for day 5. This data is presented graphically in the supplemental document, Additional file 1: Figure S6. ASCs, adipose-derived mesenchymal stromal cells; biglycan; COL, collagen; COX, cyclooxygenase; DCN, decorin; IL-1β, interleukin-1β; MMP, matrix metalloproteinase; SCX, scleraxis; TFs, tendon fibroblasts; TNF, tumor necrosis factor; TNMD, tenomodulin.
