Figure 5
Effect of control mouse mesenchymal stem cells (mMSCs) or β-catenin-overexpressing mMSCs on lipopolysaccharide (LPS)-induced lung permeability. (A) Lung oedema was measured using the ratio of lung wet weight to body weight (LWW/BW). The results are shown for samples taken 3, 7 and 14 days after LPS exposure. (B) Total protein (TP) and (C) albumin (ALB) concentration in bronchoalveolar lavage fluid (BALF) were measured using mouse-specific enzyme-linked immunosorbent assays to evaluate the epithelial permeability of the lung. (D) Expression of the occludin protein in the lung tissue on day 14 after mMSC administration was evaluated using Western blotting analysis. β-actin was used as an internal control. (E) Keratinocyte growth factor (KGF) concentration in BALF was measured using a mouse-specific enzyme-linked immunosorbent assay. The data represent the mean ± standard deviation of six animals at each time point per group. *P < 0.05, versus normal saline (NS) + phosphate-buffered saline (PBS) group; # P < 0.05, versus LPS + PBS group; & P < 0.05, versus LPS+ mMSC control group).