Influence of vascular endothelial growth factor stimulation and serum deprivation on gene activation patterns of human adipose tissue-derived stromal cells

Table 2 Expression levels for regulated genes according to primary gene ontology function

		Serum-deprived			VEGF-stimulated
Gene ID	Gene name	FC	2 ^\|FC\|		FC	2 ^\|FC\|
*Adhesion*
CLSTN2	Calsyntenin 2	+2.375		+5.187	+2.456		+5.488
JAM2	Junctional adhesion molecule B	+1.079		+2.112	NS		NS
ITGB3	Integrin, beta 3	−0.709		−1.635	−1.040		−2.056
PODXL	Podocalyxin-like protein 1	−1.126		−2.183	−1.066		−2.093
*Extracellular matrix*
SMOC2	SPARC related modular calcium binding 2	+2.059		+4.167	+2.185		+4.548
SPON1	Spondin 1, F-spondin, extracellular matrix protein	+1.120		+2.174	+1.600		+3.039
ADAMTS12	ADAM metallopeptidase with thrombospondin type 1 motif	+1.136		+2.198	+1.213		+2.318
SGCD	Sarcoglycan delta	+1.027		+2.038	+0.989		+1.985
DPT	Dermatopontin	+0.978		+1.970	+1.006		+2.008
MFAP4	Microfibrillar-associated protein 4	+0.950		+1.933	+0.964		+1.951
PTPRB	Protein tyrosine phosphatase, receptor type, B	NS		NS	−0.9992		−1.999
TGM2	Transglutaminase 2	−1.063		−2.090	NS		NS
MMP3	Matrix metallopeptidase 3	−1.493		−2.816	−1.499		−2.826
COL13A1	Collagen, type XIII, alpha 1	−1.1227		−2.178	−0.9474		−1.028
MMP1	Matrix metallopeptidase 1	−2.139		−4.405	NS		NS

Groups defined by Gene Enrichment Analysis linked with Kyoto Encyclopedia of Genes and Genomes library and gene ontology terms. Fold changes (FC) and consequently calculated amount of up (+) or down (−) regulation (2^|FC|) of significant genes (P < 0.05) compared to adipose tissue-derived stromal cells (ASCs) in complete medium are listed for vascular endothelial growth factor (VEGF)-treated and serum-deprived ASCs FC >0.9. Most of the regulated genes are shared between groups, but have been assigned to the primary group for the sake of clarity. NS, not significant.

ISSN: 1757-6512